Morphological and growth characteristics of primary cell cultures derived from philippine native banaba and rhode island red chicken (Gallus gallus domesticus l.)

Abstract

© 2019, University of the Philippines at Los Banos. All rights reserved. Primary cell cultures of chicken embryo fibroblasts derived from Philippine native Banaba chicken (BNB) and Rhode Island Red (RIR) chicken (Gallus gallus domesticus) were successfully established using the warm trypsin culture method. Cell morphology, viability, conuence, and dynamic growth were observed. The results showed that the extracted cells for both treatments were healthy exhibiting characteristics of fusiform and fibrous structure, reaching 95% conuence at 72 hr. The population doubling time ranged from 60 to 63 hr. Four passages of primary cells of BNB were successfully conducted. The cells in the second passage were preserved with 97.3% cell viability via cell cryogenic technology. The growth curve of the cells projected a typical “S” shape. T-test for two independent means (p<0.05) showed that there were no significant differences in the mean cell concentration and cell viabilities between RIR and BNB cultures. Microscopic analysis of the culture showed that there was no apparent microbial contamination. Cell culture of Banaba chicken fibroblast cells can provide genetic resources for future genomic studies. In addition, the methodology applied in this study proved to be reproducible and can be used as basis for other applicable researches.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

47-56

Document Type

Article

Subject

Banaba, Chicken embryo fibroblasts, Gallus gallus domesticus, Primary cell culture, Rhode Island Red

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