Culture storage age and fungal re-isolation from host-tissue influence Colletotrichum spp. virulence to pepper fruits
Abstract
© 2019 Blackwell Verlag GmbH Using resistant cultivars is the most sustainable and practical approach against plant diseases. Plant germplasm and breeding lines are selected and assayed against, usually, the most aggressive or virulent strains of a pathogen (e.g., fungus) that causes the disease. However, prolong storage of the pathogen in culture media could affect virulence that, consequently, also influence the outcome of the resistance assay. This study demonstrates that long-term storage (at least a year) of Colletotrichum truncatum and C. scovillei, causal agents of pepper anthracnose, in potato dextrose agar (PDA) medium decreased the aggressiveness and virulence of the fungus in host-pepper fruits. However, reintroduction of the pathogen to the host and isolation of the pathogen as the new inoculum, prior to inoculation assays, increased the virulence of the fungi. These findings suggest that re-inoculation and re-isolation of Colletotichum truncatum and C. scovillei that have been stored for at least 1 year in PDA medium are necessary when using fungal cultures in pathogenicity and plant resistance assays to achieve desirable, comparable and reliable results.
Source or Periodical Title
Journal of Phytopathology
ISSN
9311785
Page
510-515
Document Type
Article
Subject
aggressiveness, anthracnose, chilli, plant resistance screening
Recommended Citation
Balendres, Mark Angelo; De Torres, Rachele; and Dela Cueva, Fe, "Culture storage age and fungal re-isolation from host-tissue influence Colletotrichum spp. virulence to pepper fruits" (2021). Journal Article. 833.
https://www.ukdr.uplb.edu.ph/journal-articles/833