Application of antagonistic microorganisms to control tree seed and seedling pathogens

Professorial Chair Lecture

UPLB Alumni Association Professorial chair Lecture

Place

College of Forestry and Natural Resources UP Los Banos, College, Laguna

Date

2021

Abstract

The antagonistic and pathogenic microorganisms were isolated from seeds and seedlings from the field and forest nurseries.

The dual or paired culture test indicated that some antagonistic isolates effectively suppressed the growth of pathogens by direct parasitism or by the production of toxic chemical.

The antagonistic fungi inoculated to seeds did not significantly reduce percent germination. Some isolates such as Trichoderma vine, Trichoderma hanicmum and Penicillum sp. increased germination as compared to the control. The antagonistic bacteria reduced germination of seeds except Bacillus subtilis with 65% and the control with 61.25% germination. .

The result on the effectiveness of antagonistic microorganisms to control seed-pathogens in the greenhouse test showed that out of the five (5) antagonistic microorganisms (3 fungi and 2 bacteria), Trichoderma harzianum was the most effective to control six (6) seed pathogens out of the eleven (11 ) tested as compared to the performance of fungicides, Captan and Benlate. Trichoderma aureoviride was the second most effective, although it did not control the four (4) pathogens, Fusarium solani, Fusarium semitectum, Pestalotia sp. and Rhizopus stolonifer.

The growth and development of the antagonistic fungi were favored by alternating light and dark conditions, acidic medium and a temperature ranging from 20 to 30°C.

Location

UPLB Main Library Special Collections Section

Call Number

Sp. Col.

Pages/Collation

24 leaves

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