Elisa and other methods in monitoring vaccination response to hemorrhagic septicemia

Professorial Chair Lecture

Metro Manila Commission Diamond Jubilee Professorial Chair Lecture

Place

CVM-IAS Communal Building, UPLB, College, Laguna

Date

12-8-1994

Abstract

Two groups each of 15 buffaloes were vaccinated subcutaneously or intramuscularly with a PasteurelIa multocida vaccine consisting of crude capsular antigen. No significant differences in immune response were observed between the two groups using the indirect haemagglutination assay. Sera from the vaccinated buffaloes were subjected to the passive mouse protection test and there was an overall average survival rate of 87.5% when the mice were challenged with 1OOLD00 of the homologous vaccine strain.

In a second trial, 67 buffaloes were grouped into 7 groups. Six of the groups were vaccinated with whole cell or crude capsular antigen respectively combined with water, alum or an oil adjuvant respectively. The seventh group (group VII) was not vaccinated and served as the control. Sera collected from these groups were tested by an enzyme-linked immunosorbent assay (ELISA) and the results obtained analyzed by Duncan's multiple range test. The antibody responses of vaccinated groups were statistically different to the control group. Serum from these buffaloes were grouped according to their ELISA optical density values (x dilution factor) and inoculated into mice. One hundred percent protection was provided by sera with an ELISA titre between 71-90 collected 3 months after vaccination while 11 months after vaccination 100%. protection was provided but only with sera having ELISA titres greater than 90.

Location

UPLB Main Library Special Collections Section

Call Number

PL

Pages/Collation

9 leaves

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