Biologically active invertebrate lectins

Professorial Chair Lecture

Guy Potter Benton Professorial Chair Lecture

Place

Institute of Chemistry, CAS, UPLB, College, Laguna

Date

6-24-2003

Abstract

The biological activity of invertebrates Pomacea canaliculata, Holothuria sp., Holothuria atra and Synapta maculata were assayed using several methods. The bioassay were used were neutrophil migration, cytokine assay for immunomodulatory activity, cytotoxicity assay using the tetrazolium test, mitogenic activity assay, antimicrobial activity using the filter paper disc method, brine shrimp assay, and seed germination inhibition activity assay.

All the invertebrate lectins exhibited biological activities. Pomacea canaliculata and Holothuria sp lectins were found active against mouse Sarcoma T180 and A546 (human lung cancer cells). The mouse SarcomaT180 cancer cells were more susceptible to the action of both lectins as indicated by its lower IC50 value than the human A549 cells.

All invertebrate lectins exhibited mitogenic activity. However, only Synapta maculata lectin exhibited weak antimicrobial activity. Holothuria atra lectin was the only lectin used for brine shrimp assay and seed germination inhibition activities. The lectin completely inhibited germination of radish seeds.

Location

UPLB Main Library Special Collections Section (USCS)

College

College of Arts and Sciences (CAS)

Language

English

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