Microbiological control of aflatoxigenic fungi and other organisms in foods

Professorial Chair Lecture

Metro Manila Commission Diamond Jubilee Professorial Chair Lecture

Place

Institute of Food Science and technology, UP Los Banos, College, laguna

Date

6-21-1995

Abstract

Studies were conducted to control aflatoxigenic fungi and some food pathogens using other microorganisms. Cladospoium filvuminhibited growth of Aspergillus parasiticus on artificial recovery media with peanut, rice and corn infusions as well as whole grains, fish and meat. High moisture content and relative humidity adversely affect the ability of Q. NIA, culture broth in inhibiting the growth of a. strasiticus in peanut kernels. In fish, smoked herring (Sardinella sp.) treated with 100X Q. tulvum culture broth had the lowest mold count during the first day of storage. Cladosporium fulvum culture broth can help preserve smoked herring for approximately 14 days. Cladosporium fulvum culture broth shaken for 4 days completely inhibited S. typhimurium and S. groom when the culture broth comprised 70% and 60% of the mixture respectively. Inhibition becomes apparent at 10% level. Cladosoorius fulviie culture broth shaken for 4 days completely inhibited S. tYPhimUrium and 6. aVreus when the culture broth comprised 70Y. and 60% of the mixture respectively. Inhibition becomes apparent at 10% level. Cladosporium fulvum culture broth changed the natural color and odor of peanuts, rice, corn, smoked herring and meat. Efforts should be undertaken to reduce such effects. Toxicological studies using the chick embryo bioassay showed no harmful constituent in various extracts from C. fulvum. Likewise, the results of the mice assay also confirm that C. fulvum was nontoxic. No difference was noted in the physical condition of both C. fulvum treated and untreated mice. Cytotoxicity test also show that the C. fulvum culture filtrate

2 has no detrimental effect in the 3 cell lines tested. The possible mechanism of inhibition of A. parasiticus by C. fulvum was studied. AeSersIllus parasitism, exposed to the culture filtrate from C. fulvum resulted in adverse morphological changes like thinning and deformation of hyphae, reduction of spore count and size, spore disintegration and impaired spore germination. Isolation and partial characterization of the inhibitory compound elucidated by C. fulvum indicate that the active compound has a molecular weight (MW) of 274 and a molecular formula of C,.H_ 0

Location

UPLB Main Library Special Collections Section

Call Number

Sp. Col.

Pages/Collation

77 leaves

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