Isolation and screening of lipid-producing green algae and diatoms from selected lakes and rivers within Laguna, Philippines

Date

2023

Abstract

Field sampling in selected lakes and rivers within Laguna [Philippines] area and laboratory experiments in UP Los Baños were conducted from April 2008 up to July 6, 2009 with the aim of isolating and screening green algae and diatoms with fast growth rate and high lipid content for future use in local microalgal biodiesel production. A collection of 30 isolates were successfully purified and grown. The isolates belong to the following species: Chlorella vulgaris (14), Chlorococcum humicola (9), Ankistrodesmus falcatus (1), Scenedesmus quadricauda (2), Nitzschia plaea (3) and Navicula minima (1). The fastest growth was observed in C. vulgaris Cv1 isolate at 2.3313 days doublings time (T2) for the 28 day indoor cultivation in a 6 L plastic vessel. The highest neutral lipid content was also observed in the same isolate with a value of 3.41% using n-hexane at 10 ml per gram of the dried biomass. To be comparable with the literature values of 10-30% total lipid for C. vulgaris, the researcher conducted a second lipid extraction procedures using 2:1 Chloroform-methanol solution at a solvent ratio of 20 mL per gram of dried biomass. The highest total lipid content after combining the yield in the 1st and 2nd extraction is still C. vulgaris Cv1 with a value of 23.78%. The top 10 isolates selected based on total lipid content and growth parameters were recommended for further studies. The Cv1 isolate was the one used by the researcher in the DOST-PCAMRD funded project entitled 'Biodiesel feedstock production from freshwater micro-algae' from July 2009-June 2011.

Language

English

Document Type

Article

Pages /Collation

25 leaves

En – AGROVOC descriptors

ALGAE; LIPID CONTENT; BIOFUELS; BACILLARIOPHYCEAE; LAKES; RIVERS; ENERGY RESOURCES; ISOLATION; PHILIPPINES

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