Isolation and characterization of larvicidal compounds from bayati [Anamirta cocculus (Linn.)] and evaluation of toxicity against mosquito (Aedes aegypti)larvae
Date
7-2015
Abstract
The larvicidal activity of bayati (Anamirta cocculus Linn.) seeds against Aedes aegypti larvae was determined prior to extraction and was found to cause percent mortality of 90.83 +- 5.77% and 100.00% +- 0.00% 24 HAT and 48 HAT, respectively at 5000 mg/L. Phytochemical screening of powdered seeds indicated presence of alkaloid, terpenoid and saponin. Extraction of pulverized bayati seeds using ethanol produced a yellow brown extract with 38.705% yield. Larvicidal toxicity assay of crude ethanolic extract showed lower percent mortality against A. aegypti compared to black pepper (botanical insecticide) and deltamethrin (synthetic insecticide). Fractionation and purification of the crude extract by silica gel column chromatography yielded four isolates: 1 (0.52% yield), 2 (1.15% yield), 3(0.67% yield) and 4 (0.32% yield). Larvicidal assay revealed that isolate 1 [LC sub 50 = 313.54 mg/L 24 hours after treatment (HAT)], 2 (LC sub 50 = 273.58 mg/L 24 HAT) and 3 (LC sub 50 631.17 mg/L 24 HAT) can be considered weakly effective while isolate 4 (LC sub 50 = 1000.40 mg/L 24 HAT) as nontoxic against A. aegypti larvae. Melting point, FT-IR and sup 1 H-NMR spectral analyses suggest that isolate 3 could be picrotoxinin and Isolate 4 could be picrotin. In addition, ethanolic extract of bayati was found to be non-toxic against A. aegypti larvae.
Language
English
Document Type
Article
Recommended citation
Abrera, A. T., "Isolation and characterization of larvicidal compounds from bayati [Anamirta cocculus (Linn.)] and evaluation of toxicity against mosquito (Aedes aegypti)larvae" (2015). Report. 1059.
https://www.ukdr.uplb.edu.ph/reports/1059
Pages /Collation
36 leaves
En – AGROVOC descriptors
AEDES AEGYPTI; CULICIDAE; ACETYLCHOLINESTERASE; LARVAE; INSECT CONTROL; EXTRACTS; SEEDS; BOTANICAL INSECTICIDES; ISOLATION; TOXICITY