Multilocation performance evaluation of a new banana bunchy top virus (BBTV)-resistant lakatan cultivar

Date

2016

Abstract

Banana bunchy top virus (BBTV) is the most destructive virus disease of banana in the Philippines. Incorporation of resistance to BBTV by conventional breeding is not possible due to sterility of most commercial banana cultivars. Using gamma irradiation and in vitro-induced mutagenesis, promising 'Lakatan' mutant lines with intermediate resistance to BBTV and aphid vector (Pentalonia nigronervosa) were selected, evaluated and characterized, and the genetic stability of BBTV resistance confirmed in succeeding generations (PCAARRD-GIA project 1999-2005, 2007-2010). The project 'Multi-Location Performance Evaluation of A New Banana Bunchy Top Virus (BBTV) – Resistant 'Lakatan' Cultivar' (2013-2016) aimed to make 'Lakatan' banana production more profitable to small farmers by reducing losses due to banana bunchy top virus infection by 20% through adoption of disease resistant Lakatan. Prior to commercialization of the five selected improved 'Lakatan' mutant lines, the agronomic, yield, and economic performance, and the variability of host (mutant lines) reaction to BBTV isolates/strains were assessed in several locations across the country. The disease reaction o f 'single' mutant line and 'bulk' or composite of the five mutant lines were assessed to determine the best planning strategy for sustainable BBTV control and management. Promotion and dissemination of the 'Lakatan' mutant lines through farmers' demonstration trials, farmers' field days, trainings and seminars were conducted for increased awareness, wider acceptance and utilization of the improved 'Lakatan' mutant line varieties. The five selected BBTV resistant Lakatan mutant lines (ML 13-20-2, 28-30-2, 22-28-2,9-28-2, 9-28-3) were evaluated in replicated multilocation trials in five Lakatan growing areas in Region 4 (Laguna, Batangas, Cavite), Region 2 (Quirino) and Region 11 (Davao City). The mutant lines were likewise evaluated in several farmers' field using farmers' practices. Variations in agronomic characteristics and yield parameters of Lakatan mutant lines and control were observed across the different sites. Bunch yield of mutant lines across locations ranged from 9.2 to 16 k/bunch in Region 4, and 17.7 to 20.3 kg/bunch in Region 11. The control Lakatan had a mean yield 10.9 to 17.9 kg/bunch. In trial most sites, BBTV resistant improved Lakatan had higher mean bunch yield compared with the control Lakatan and the highest mean yield performance was observed in Davao City. Under farmers' field, bunch yield range from 10 to 18 kg/bunch. The BBTD incidence from planting up to the first harvest of mutant lines across locations ranged from 1.36% to 33.3% while control Lakatan had 17 to 50% incidence. In all trial sites mutant lines had lower disease incidence than Lakatan control. The highest BBTD incidence was observed in Cavite province which is a known hotspot for BBTV. The spread of BBTD was likewise slower in BBTV resistant mutant lines compared to the ordinary control Lakatan. To study the variability of disease, BBTV-infected plant samples were collected from nine provinces all over the country.

Language

English

Document Type

Article

Pages /Collation

99 leaves

En – AGROVOC descriptors

MUSA (BANANAS); VARIETIES; DNA; GENES; EVALUATION; AGRONOMIC CHARACTERS; YIELDS; NUCLEOTIDE SEQUENCE; GENOMES; BANANA BUNCHY TOP VIRUS; GENETIC STABILITY; DISEASE CONTROL; CROP PERFORMANCE; DISEASE RESISTANCE; MUTANTS; BREEDING METHODS; MORBIDITY

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