Molecular characterization and infectivity of the cotton leaf curl virus isolated from gumamela (Hibiscus rosa-sinensis) in the Philippines

Date

2012

Abstract

Hibiscus rosa-sinensis or gumamela at the Institute of Plant Breeding (IPB) were observed showing typical symptoms of the leaf curl disease like cupping or curling of the leaves, vein enation and clearing, mottling and stunting. Transmission tests were done using indicator hosts to isolate and determine the host range of the putative virus. Leaf cupping, veinenation, mottling and mosaic were observed in Gossypium hirsutum (cotton) and Vigna unguiculata (cowpea) 1 month after inoculation using whiteflies. Thirty four out of the 40 collected gumamela samples showed the expected band of approx 1.5kb after PCR amplification using degenerate primers, thereby confirming the presence of a begomovirus. The isolates were further characterized by sequencing, multiple sequence alignment and phylogenetic analysis. Multiple sequence alignment of the obtained DNA-A fragments revealed 97.0-99.0% similarities among isolates. Furthermore, sequence analysis using other leaf curl virus sequences in the GenBank showed that the begomovirus infecting gumamela in the Philippines was highly identical to the Cotton leaf curl Multan virus with 98% nucleotide similarities. Phylogenetic analysis showed that CLCuV isolates in the Philippines clustered with the CLCuV isolates from the other countries which were also isolated from Hibiscus plants as well as cotton and okra. Furthermore, DNA betha is detected in CLCuV-infected gumamela plants as well as other genetic components which may be essential factors in disease complexes.

Language

English

Document Type

Article

Pages /Collation

16 leaves

En – AGROVOC descriptors

HIBISCUS ROSA SINENSIS; LEAVES; LEAF CURLS; COTTON; PLANT DISEASES; PLANT VIRUSES; PCR; DNA

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