Development of nanosensors and nanostructured materials from agricultural by products for enhancement of food and agricultural productivity and for environmental sensing and remediation: Bench-scale production of nanosensors for the detection and analysis of arsenic in contaminated water-development of a fluorescent nanobiosensor for the detection of arsenic
Date
2016
Abstract
The authors developed an electrochemical nano-biosensor for arsenic detection using an engineered bacterial periplasmic binding protein in a carbon paste electrode. The protein and its mutant having four more cysteine residues were compared in terms of arsenic binding by fluorescence quenching. The mutant had slightly better response in the presence of arsenic. The mutant protein was immobilized as a cross-linked protein aggregate and then used as modifier of a carbon paste electrode. Cyclic and differential pulse anodic stripping voltammetry analyses showed peaks that could be due to arsenic. At this stage, the authors were able to detect a response at arsenic concentrations of at least 10 ppm.
Language
English
Document Type
Article
Recommended citation
Recuenco, M. C. and Peralta, M. M., "Development of nanosensors and nanostructured materials from agricultural by products for enhancement of food and agricultural productivity and for environmental sensing and remediation: Bench-scale production of nanosensors for the detection and analysis of arsenic in contaminated water-development of a fluorescent nanobiosensor for the detection of arsenic" (2016). Report. 435.
https://www.ukdr.uplb.edu.ph/reports/435
Pages /Collation
67 leaves
En – AGROVOC descriptors
WATER; ARSENIC; ARSENIC COMPOUNDS; CYSTEINE; RESIDUES; FLUORESCENCE; TOXICITY; VOLTAMMETRY; ELECTRODES; BIOSENSORS