Title

In vitro assessment and identification of fungi isolated from the rhizospheres on agricultural crops associated with vesicular arbuscular mycorrhizal (VAM) inoculant

Date

2013

Abstract

Enhancement and development of effective plant growth promoting inoculants demand the presence of different set of attributes which can help in colonizing the rhizosphere. Fungi from the roots and soil rhizospheres of different crops from VAM inoculants are isolated and screened.Isolates that showed a positive results in phosphate solubilization were tested in vitro on IAA production assay. The total phosphate content available in the culture filtrate was measured by UV-vis spectrophotometer at 420 nm following the vanadate molybdate method while for IAA production assay, colorimetric determination using Salkowsky's reagent. The result showed the highest solubilisation of Tricalcium Phosphate (TCP) in Pikovskaya's liquid medium, however, no isolates positively response in IAA production assay. Identification of isolates was done in the basis of cultural and morphological characterization. Fungal microorganisms which showed good phosphate solubilisation are considered as the best candidates for the development of biofertilizer.

Book Title

Biofertilizer technologies for sustainable agriculture

Language

English

En – AGROVOC descriptors

IN VITRO; FUNGI; VESICULAR ARBUSCULAR MYCORRHIZAE; ROOTS; RHIZOSPHERE; BIOFERTILIZERS; PHOSPHATES; SOLUBILIZATION

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