Morphological and haplotype analyses of Zinc deficiency tolerance in the somaclonal variants of "Pokkali" rice (Oryza sativa L.)

Date

6-2018

Abstract

Four somaclonal variants of Pokkali were screened for zinc (Zn) deficiency tolerance and other agronomic traits at early vegetative stage using hydroponic solution and at mid-vegetative stage grown in Zn deficient soil. The samples were genotyped using 24 SSR markers and the 6k Beadchip of Infinium 6k SNP genotyping platform. Significant differences were observed among genotypes for zine deficiency tolerance and other agronomic traits. Shoot Zn concentrations dropped below the critical level of 15 g Zn g-1 for all somaclonal variants tested including check varieties. Haplotype analysis revealed that somaclonal variants were found to have resemblance with the alleles present in Pokkali, although none of them had all alleles present in the reference genotype. Somacional variants and Pokkali shared a total of 104 SNPs. Furthermore, SSR marker haplotypes and SNPs revealed that somaclonal variants were indeed derived from Pokkali. However, these markers cannot be associated with the observed zinc deficiency tolerance and susceptibility.

Document Type

Master Thesis (Limited)

College

Graduate School (GS)

Adviser/Committee Chair

Teresita H. Borromeo

Committee Member

Ma. Genaleen Q. Diaz, Glenn B. Gregorio

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

En – AGROVOC descriptors

ORYZA SATIVA; SOMACLONAL VARIATION; ZINC; DEFICIENCIES; TOLERANCE; MORPHOLOGY; HAPLOTYPES

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