Morphological and haplotype analyses of Zinc deficiency tolerance in the somaclonal variants of "Pokkali" rice (Oryza sativa L.)
Date
6-2018
Abstract
Four somaclonal variants of Pokkali were screened for zinc (Zn) deficiency tolerance and other agronomic traits at early vegetative stage using hydroponic solution and at mid-vegetative stage grown in Zn deficient soil. The samples were genotyped using 24 SSR markers and the 6k Beadchip of Infinium 6k SNP genotyping platform. Significant differences were observed among genotypes for zine deficiency tolerance and other agronomic traits. Shoot Zn concentrations dropped below the critical level of 15 g Zn g-1 for all somaclonal variants tested including check varieties. Haplotype analysis revealed that somaclonal variants were found to have resemblance with the alleles present in Pokkali, although none of them had all alleles present in the reference genotype. Somacional variants and Pokkali shared a total of 104 SNPs. Furthermore, SSR marker haplotypes and SNPs revealed that somaclonal variants were indeed derived from Pokkali. However, these markers cannot be associated with the observed zinc deficiency tolerance and susceptibility.
Document Type
Master Thesis (Limited)
College
Graduate School (GS)
Adviser/Committee Chair
Teresita H. Borromeo
Committee Member
Ma. Genaleen Q. Diaz, Glenn B. Gregorio
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Recommended Citation
Calvo, Cris Harvin Rey G., "Morphological and haplotype analyses of Zinc deficiency tolerance in the somaclonal variants of "Pokkali" rice (Oryza sativa L.)" (2018). Graduate Student's Output. 2969.
https://www.ukdr.uplb.edu.ph/etd-grad/2969
En – AGROVOC descriptors
ORYZA SATIVA; SOMACLONAL VARIATION; ZINC; DEFICIENCIES; TOLERANCE; MORPHOLOGY; HAPLOTYPES