Influence of age and length of temephos exposure on lipofuscin and hemosiderin proliferation in the splenic Melanomacrophage Centers (MMCs) of the nile tilapia (Oreochromis niloticus L.)
Date
4-2010
Degree
Bachelor of Science in Biology
Major Course
Major in Zoology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Pablo P. Ocampo
Abstract
The study aims to determine the influence of age and length of temephos exposure on lipofuscin and hemosiderin proliferation in splenic melanomacrophage centers (MMCs) of Oreochromis niloticus. Age-related and time-course experiments were conducted separately. Thirty, 1 ½ month old individuals were equally distributed to 3 age groups which are the following: 2, 3, and 4 months. On the other hand, fifty, 3 month old individuals were subjected to 5 time-course treatments which include: 3, 7, 11, 15 days and control (untreated for 15 days). Low concentration of temephos (0.16mg/L) was applied. Lipofuscin and hemosiderin pigments were measured using Image J analysis program. One way ANOVA showed significant differences among the relative spleen weight of the age groups (F= 11.575). Likewise, relative spleen weights of time-course treatments obtained significant differences (F= 3.013). Kruskal- Wallis ANOVA showed no significant differences in lipofuscin size among age groups (KW=2.220, P0.05). Hemosiderin areas of age (KW=4.340, P>0.05) and of pesticide-treated groups (KW=8.108, P>0.05) were found to be not significantly different with each other. Lipofuscin compositions (%) among age groups (KW= 4.860, P>0.05) and among treated groups (KW= 6.860, P>0.05) did not vary significantly among each other. The study showed that pesticide contamination causes lipofuscin proliferation. In contrast, age and toxicant exposure did not influence the hemosiderin size.
Language
English
Location
UPLB Main Library Special Collections Section
Recommended Citation
Biel, Darahlyn M., "Influence of age and length of temephos exposure on lipofuscin and hemosiderin proliferation in the splenic Melanomacrophage Centers (MMCs) of the nile tilapia (Oreochromis niloticus L.)" (2010). Undergraduate Theses. 1.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1
Document Type
Thesis