Date

4-2010

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Pablo P. Ocampo

Abstract

The study aims to determine the influence of age and length of temephos exposure on lipofuscin and hemosiderin proliferation in splenic melanomacrophage centers (MMCs) of Oreochromis niloticus. Age-related and time-course experiments were conducted separately. Thirty, 1 ½ month old individuals were equally distributed to 3 age groups which are the following: 2, 3, and 4 months. On the other hand, fifty, 3 month old individuals were subjected to 5 time-course treatments which include: 3, 7, 11, 15 days and control (untreated for 15 days). Low concentration of temephos (0.16mg/L) was applied. Lipofuscin and hemosiderin pigments were measured using Image J analysis program. One way ANOVA showed significant differences among the relative spleen weight of the age groups (F= 11.575). Likewise, relative spleen weights of time-course treatments obtained significant differences (F= 3.013). Kruskal-Wallis ANOVA showed no significant differences in lipofuscin size among age groups (KW=2.220, P0.05). Hemosiderin areas of age (KW=4.340, P>0.05) and of pesticide-treated groups (KW=8.108, P>0.05) were found to be not significantly different with each other. Lipofuscin compositions (%) among age groups (KW= 4.860, P>0.05) and among treated groups (KW= 6.860, P>0.05) did not vary significantly among each other. The study showed that pesticide contamination causes lipofuscin proliferation. In contrast, age and toxicant exposure did not influence the hemosiderin size.

Language

English

LC Subject

Zoology

Document Type

Thesis

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