Quantification of embryonic development of honey bees (Apis mellifera L.) at different temperatures

Date

4-2012

Degree

Bachelor of Science in Applied Physics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lou Serafin M. Lozada

Abstract

The number of cells and rate of mitotic cell division at different parts of a honey Lk bee (Apis mellifera L.) embryo were measured by associating the number of cells IF in the embryos to the intensity of the absorbed stain. Using a standard staining method, the intensity of the absorbed stain was monitored every 2 hours. Image _1 analysis using ImageJTM was performed and an intensity profile for the anterior-posterior axis of each embryo was plotted. The same procedure was implemented for embryos kept at 35 ± 1 °C (control), -7 ± 1 °C and 42 ± 1 °C. Results showed that the number of cells and the rate of cell division are highest at the anterior (head) part for all three temperatures. The position of the peaks (highest intensity) of the anterior (head) and posterior (tail) part remained between 0.20 ± 0.01 to 0.24 ± 0.01 and 0.80 ± 0.01 to 0.85 ± 0.01 of the fractional egg length respectively. Based on the intensity profiles, the number of cells of the embryos kept at —7 ± 1 °C is lower compared to that of 35 ± 1 °C and 42 + 1 °C. The number of cells of embryos kept at 42 ± 1 °C is highest among all three temperatures, but on the third day, no apparent difference in the intensities was observed within the 8 hour period. The embryos kept at -7 ± 1°C and 42 ± 1 °C did not continue to develop into larvae unlike that of 35 ± 1 °C. It was concluded that the number of cells and the rate of cell division of honey bee embryos were significantly different at extreme temperatures.

PACS: 87.17;d, 87.15.Vv, embryogenesis, quantification, honey bee

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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