Isozyme variation in populations of Locusta migratoria manilensis meyen (Orthoptera: Acrididae) from Tarlac and Pampanga.

Date

4-1996

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

The isozyme variability in populations of Locusta migratoria manilensis Meyen from Tarlac and Pampanga were compared and analyzed using Mupid2 system starch gel electrophoresis. Esterase (Est) and alkaline phosphatase (Alph) were the two loci observed. Alph exhibited polymorphism and was found to be controlled by a single locus with two alleles, AlphlF and Alph1S. Chi-square test showed a goodness-of-fit of the observed to the expected gene frequencies under Hardy-Weinberg equilibrium only for Pampanga population. Esterase was found to be controlled by three loci. Estl is monomorphic, while Est2 and Est3 is polymorphic with two allozymes each. The goodness-of- fit was tested by using the chi-square method and results showed that the observed genotype proportions for both populations do not fit the expected proportions under Hardy-Weinberg equilibrium. The genetic identity of the two populations was 0.9672 and genetic distance was 0.0333. This shows that the alleles and gene frequencies of the two populations are almost identical which implies that the two populations could have come from one initial outbreak area which developed as swarms.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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