Preliminary studies on the in vitro culture of fibroblasts in coconut water medium.

Date

3-1980

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

Trypsin-dissociated lung fibroblasts of new-born mice were cultured for 72 hours in different serum supplemented media - Pure hank's Basal Salt Solution (HBSS), varying ratios of coconut water with HBSS and pure coconut water. In both pure HBSS and pure coconut water media: cells maintained their histotypic characteristics they were able to aggregate into colonies and eventually into monolayers. Results showed that cell division and protein synthesis rates increased as more parts of coconut water were added with HBSS. Hence, cell number and cell protein content were highest in pure coconut water culture than in pure. HBSS culture. However, such was only true Curing the first 48 hours of culture, because curing the 72nd hour, the amount of cell protein synthesized and the number of cells added to previous population were minimal in coconut water cultured fibroblasts and maximal in HBSS-cultured fibroblasts. Dye exclusion test for cells' viability showed that serum-supplemented coconut water offered a better environment that can maintain cells in a viable state than serum-supplemented HBSS. The reasons behind these observations were discussed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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