A study on the use of coconut water as a chromosome medium for cytological studies

Date

4-1984

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M Engle

Abstract

Coconut water fortified with horse serum and phytohemagglutinin crude extract was used as a medium for human leukocytes. the pH of the medium was adjusted to approximately 7.2 with 7% sodium bicarbonate buffer. the cells were cultured for 72 hours at 37°C.

Cell viability in coconut water was maintained, however, its performance was inferior as compared to the standard chromosome medium. no metaphase cells were apparent, although a few cells at the prophase stage pf cell division were evident.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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