Quantitative Trait Loci (QTL) analysis and mapping of female and male fertility genes in an interspecific cross of tomato (Lycopersicon esculentum Mill. X Lycopersicon pimpinellifolium (Jusl.) Mill.).

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Quantitative traits related to female fertility were mapped on the chromosomes of the recombinant inbred lines of a cross between cultivated tomato (Lycopersicon esculent= Mill.) and currant tomato (Lycopersicon (Jusl.) Mill.). Morphological characteristics related to fertility were observed. Most of the fertility traits observed have values near that of L. pimpinellifolium parent. At a<0.05, a significant weak association was observed for pollen number and number of seeds per fruit. On the other hand, at a<0.01, very weak associations were detected for fruit weight and stigma exertion; and fruit weight and pollen number. Forty-one Simple Sequence Repeats (SSR) markers and two Single Nucleotide Polymorphism (SNP) markers were used to saturate the previous genetic map for the LAl269 RILs. A total of sixteen markers (14 SSR and 2 SNP) were incorporated into the genetic map of the same population. The phenotypic and genotypic data gathered were used in the QTL analysis. A total of 11 QTLs related with fertility with LOD scores greater than 2.0 was detected. These include the following: one fruit weight QTL which is located at chromosome 7; one for pollen number at chromosome 11; four for pollen viability at chromosomes 4, 5, 6, and 9; two for number of seeds per fruit both at chromosome 3; one for number of flowers per inflorescence at chromosome 8; and two for stigma exertion at chromosomes 6 and 8.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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