Comparative electrophoretic analysis of the alkaline phosphatase banding patterns in Apis mellifera L. and Apis cerana L.

Date

6-1991

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Starch gel electrophoresis revealed variations in the alkaline phosphatase banding patterns between Apis mellifera and Apis cerana. Three zones of enzymatic activity, designated as Aphl, Aph2 and Aph3, were observed. Aph2 and Aphl were found polymorphic for A. mellifera and A. cerana respectively. Aph2 was distinct for A. mellifera, and exhibited three phenotypes (Aph2FF, Aph2FS and Aph2SS) formed by a fast and a slow band. Aphl and Aph3, on the otherhand, were distinct only for A. cerana. Aphl exhibited two banding patterns (AphlFS and Aph1SS) formed also by two bands. The third zone Aph3 had a cathodal characteristic, and limitations in the gel did not allow extensive investigation on the zone. The intermediate band in the heterozygote characterizing the dimeric structure of alkaline phosphatase was not observed in either species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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