Effects of different concentrations of lead chloride on the growth, chlorophyll a and cyto-morphology of the blue green alga, Oscillatoria willei Gardn. Em. drouet (Division cyanophyta).

Date

10-2004

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Milagrosa, R. Martinez-Goss

Abstract

The effects of different concentrations of lead chloride on the unialgal culture of the blue green alga. Oscillatoria Gardn. em. Drouet. were determined. Test concentrations were: 0ppm (T1), 5ppm (T2), 20ppm (T3). 15ppm (T4). and 20ppm (T5) of lead chloride in BG-11+1\1, a basal inorganic medium. Growth was measured by the increase in diameter of the algal colony taken every 2 days for a period of 6 days. The study showed significant increase in growth of the alga at a = 0.05 especially at 2Oppm. The treatments gave an increasing order of growth: Tl< T2< T3< T4< T5. Optimum growth rate was noted after 2 days of incubation for T5 at 1.0153 doublings per day. Chlorophyll analysis was done at the logarithmic phase of growth of the alga in all treatments. Chlorophyll concentrations showed an increasing trend as the concentration of lead increased. Chlorophyll a was highest for T5 (20ppm PbC'12) with a value of 0.5178 pg/ml. followed by T4, T3, T2. and T1 with values of 0.4605 pg/ml, 0.4319 pg/ml. 0.2112 1.1g/ml, and of 0.1712 1.1g/ml. respectively. More enhanced blue green color was observed as the concentration increased up to a level of 20 ppm of lead chloride. Constrictions in the cross walls of the trichomes were also more prominent as the concentration increased.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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