Isolation of auxotrophic and metal-resistant mutants of Saccharopolyspora erythraea ATCC 11635 and their erythromycin yield.

Date

3-1992

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Asuncion K. Raymundo

Abstract

Out of 60 randomly selected N-methyl-N-nitro-N-nitrosoguanidine treated colonies of Saccharopolyspora erythraea ATCC 11635, only one strain encoded 4-25. was found to be a potential auxotroph after five generations in Minimal Medium. This isolate, however, was prototrophic in the Holiday's Pool. To induce this strain to true auxotrophy, UV mutagenesis was done. However, no true auxotrophs were developed. As an alternative, spontaneous metal-resistant mutants were screened on plates increased concentrations of mercury, copper, and cobalt. The number of resistant mutants to each reagent were 3, 10, and 15, respectively. All metal-resistant and some selected mutagenized were assayed to determine erythromycin activity. One strain, a UV-treated isolate of 4-25 gave a significantly higher yield of 24,204 ug/ml compared to the 8.182 ug/ml of the wild type.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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