Isolation of auxotrophic and metal-resistant mutants of Saccharopolyspora erythraea ATCC 11635 and their erythromycin yield.
Date
3-1992
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Asuncion K. Raymundo
Abstract
Out of 60 randomly selected N-methyl-N-nitro-N-nitrosoguanidine treated colonies of Saccharopolyspora erythraea ATCC 11635, only one strain encoded 4-25. was found to be a potential auxotroph after five generations in Minimal Medium. This isolate, however, was prototrophic in the Holiday's Pool. To induce this strain to true auxotrophy, UV mutagenesis was done. However, no true auxotrophs were developed. As an alternative, spontaneous metal-resistant mutants were screened on plates increased concentrations of mercury, copper, and cobalt. The number of resistant mutants to each reagent were 3, 10, and 15, respectively. All metal-resistant and some selected mutagenized were assayed to determine erythromycin activity. One strain, a UV-treated isolate of 4-25 gave a significantly higher yield of 24,204 ug/ml compared to the 8.182 ug/ml of the wild type.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Arellano, Jose Alden O., "Isolation of auxotrophic and metal-resistant mutants of Saccharopolyspora erythraea ATCC 11635 and their erythromycin yield." (1992). Undergraduate Theses. 10692.
https://www.ukdr.uplb.edu.ph/etd-undergrad/10692
Document Type
Thesis