Genetic characterization of pili (Canarium ovatum Engl.) through isozyme analysis using starch-gel electrophoresis.

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

(Canarium ovatum Engl.) from the germplasm collection of the National Plant Genetic Resources Laboratory, Institute of Plant Breeding, UPLB, which originated from Oas, Albay. A total of five presumptive polymorphic loci and 11 genotypes were detected from the four enzymatic systems analyzed: Alcohol dehydrogenase (ADH), phosphogluconate dehydrogenase (PGD), phosphoglucoisomerase (PGI) and phosphoglucomutase (PGM). One locus was observed for ADH, PGD and PGI. Three genotypes were observed for ADH-1 (SS, FS and FF). Genotypes FF and SS were observed in PGD-1 and PGI-1. Two loci, PGM-1 (with genotypes SS, FS, and FF) and PGM-2 (FF and SS), and 5 band patterns were observed in PGM. Among the five polymorphic loci, the highest genotypic frequency was obtained by the FF genotype of PGD-1 (0.727), while the lowest was obtained by the SS genotype of ADH-1 (0.100). Gene F of PGD-1 exhibited the highest gene frequency (0.727), while its S gene exhibited the lowest gene frequency (0.273) The highest differentiation of alleles among the different loci (8T) (0.538) was obtained by PGM-2, while PGD-1 exhibited the lowest variation (0.437). The total population differentiation for the gene pool (6T) is equal to 0.483. Variation within the population, considering all of the samples came from the same origin, may be attributed to the dioecious nature of pili and its propagation using seeds/seedlings.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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