Confirmation of hybridity of three tomato (Lycopersicon esculentum Miller) experimental F1 hybrids using seed and leaf protein and isozyme markers.

Date

3-2002

Degree

Bachelor of Science in Biology

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and starch gel electrophoresis were employed to confirm hybridity of three F1 experimental hybrids of tomato, Lycopersicon esculentum Miller: XTH-6137, from P1 x P2; XTH-6174, from P6 x P5; and XTH-6193, from P8 x P9. Marker seed proteins were found in the staminate parents P2 and P9; while leaf protein markers were found in the staminate parents P2, P5, and P9. Results showed that the F1 samples are true hybrids of their respective parents. The similarity indices among the samples were high since the samples belong to the same species. Isozyme markers were found in esterase, malate dehydrogenase, and acid phosphatase that further proved the hybridity of the three experimental F1 samples. No marker was found in alkaline phophatase suggesting that this enzyme could not prove tomato hybridity.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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