Comparative karyotypes of two Philippine native orchids (Cleisostoma subulatum Blume and Spathoglottis plicata 'Alba' Blume.

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

Mitotic behavior, chromosome number, and chromosome characteristics were determined in two Philippine native orchids, Cleisostoma subulatum Blume and Spathoglottis plicala 'Alba' Blume using acetocarmine squash preparations of root tip cells. Both species exhibited normal mitosis with C. subulatum having a higher mitotic index of 73.72% than S. plicata 'Alba' with 63.81%. Karyotype analysis showed a difference in the diploid chromosome number: C. subulatum had a diploid number of 2n= 38 while S plicata Alba' had 2n=40. The average chromosome lengths ranged from 0.0268 to 0.1000 in C. subulatum and 0.0257 to 0.0883 in S. plicata 'Alba'. Based on centromeric position, the chromosomes in both species were either metacentric or submetacentic. In C. subulatum, chromosomes 2, 3, 8 to 11, 13 to 18 were metacentric while chromosomes 1, 4 to 7, 12 to 19 were submetacentric. Chromosomes 1, 3, 5 to 7, 9 to 19 were metacentric in S. plicala 'Alba' while 2, 4, 8, 10, 11, 15, and 20 were submetacentric. Chromosome 4 was submetacentric while chromosomes 3, 9, 13, 14, 16, 17 and 18 were metacentric in both species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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