Stigmatic protein profiles and isozyme analysis of pin and thrum morphs of Mussaenda using sodium dodecyl sulfate polyacrylamide gel electrophoresis and starch gel electrophoresis.

Date

3-2003

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Simeona V. Siar

Co-adviser

Genaleen Q. Diaz

Abstract

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and starch gel electrophoresis were employed to compare the stigmatic protein profile of the pin (long-styled) and thrum (short-styled) morphs of Mussaenda. In SDS-PAGE, similar band patterns were observed among cultivars of the same morph. Protein bands with 40kDa and 36kDa sizes were found to be unique in the pin morphs while a distinct 50kDa band was seen in all thrum morphs. `Lakambini,' whose anther and style are of the same length, was found to have the two unique bands of the pin morphs and the single band exclusively found in the thrum morphs. This further established its intermediate identity. In the analysis of isozymes, a total of six putative loci were found—one in acid phosphatase, four in esterase and one in alkaline phosphatase. Comparison of pin and thrum banding patterns in each enzyme system showed variability between and within morphs, however, a distinct EST3-1 of esterase was seen to be present exclusively in all thrum cultivars. Using the combination of the six putative loci (ACP1/(ESTUESTVEST3/EST4)/AKP1), the pin and thrum cultivars were genetically differentiated from each other based on their genotypic designations.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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