Nitrogenase activity of frankia- type actinomycete isolated from sugarcane rhizosphere and garden soil.

Date

10-1988

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ruben B. Aspiras

Abstract

Frankia-type actinomycetes (FTA) were enumerated in sugarcane rhizosphere and in garden soil from the same area in Canlubang, Laguna. They were isolated, purified and then assayed for nitrogenase activity (acetylene reduction). In addition, morphological studies were conducted on representative isolates. FTA isolates from garden soil comprised 60.2% of the total bacterial population compared to 45.9% obtained from the rhizosphere sample. The garden soil FTA population were not only more numerous but the representative isolates had higher nitrogenase activity (acetylene reduction) than their rhizosphere counterparts. Representative isolates studied morphologically showed that those active in nitrogen fixation formed colonies characterized by the presence of dark zones where hyphae containing concentration of vesicles were located. The vesicles are the sites of nitrogenase activity. The results obtained in this study indicate that continuous use of inorganic nitrogen fertilizer in sugarcane might be responsible for the decrease both in number and in nitrogenase activity of nitrogen-fixing actinomycete.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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