Genetic variation in the esterase locus of the striped stemborer Chilo suppressalis, (Walker).

Date

3-1991

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Co-adviser

Cesar G. Demayo

Abstract

Investigation of the existence of genetic variation in the esterase locus of the striped stemborer C. suppressalis was performed through electrophoresis and spectrophotometry. Vine electrophoretic phenotypes were observed in C. suppressalis and of these, the genotypes MM and MF which consisting of one band and three bands respectively were subjected to factors such as pH, Temperature, different substrates, substrate competition, inhibitors and reaction time to determine the differences between the activities of the homozygous and the heterozygous genotypes. Both genotypes had the esterase activity increased with substrate concentration, reaction time and sample amount. Optimum pH (pH 8 for both genotypes) and temperature (70•C for MF and 55"C for MM) were determined. The presence of other substrates as competitors, decreased esterase activity. Eserine, EDTA, urea and mercaptoethanol inhibited esterase activity. Thus, there exists a difference in esterase activity of the homozygous MM and the heterozygous MF genotypes, the MF genotype generally having higher esterase activity than the MM genotype.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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