Karyotype of the banana tree frog Rhacophorus leucomystax (Kuhl) (Anura: Rhacophoridae).

Date

10-1990

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Mitotic cells of 72-hour tadpoles and developing hindlimbs of fully developed tadpoles of the banana tree frog, Rhacophorue leucomystax (Kuhl), were investigated. Lacto-acetoorcein squash technique of larval tissues pretreated with 0.05% colchicine and fixed in 3:1 ethyl alcohol-glacial acetic acid revealed normal chromosome behavior during the different stages of mitosis. R. leucumystax had a diploid chromosome number of 2n=26 consisting of monocentric metacentric chromosomes classified into 5 pairs of large chromoomes and 8 smaller pairs with average relative lengths ranging from 0.068 to 0.042 and 0.038 to 0.020, respectively. Different degrees of chromosome con-traction slightly caused variations in their relative lengths, though contraction of chromosomes resulted to decreased lengths and increased diameter. A satellite was distinguished in the premetaphase chromosome number 9. The mean mitotic indices were 26.20 in 72-hour larval cells and 26.15% for hindlimb cells.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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