Isozyme analysis of selected edible mushrooms of order Agaricales

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Isozyme investigations were conducted on four edible mushroom species, namely: Volvariella volvacea (Bull. ex Fr.) Sing. from Los Baños and from Nueva Ecija, Pleurotus florida Eger, and P. sajor-caju (Fr.) Singer. They were analyzed based on four enzymes. namely, acid phosphatase (ACP), alkaline phosphatase (ALP). esterase (EST), and glutamate oxaloacetate transaminase (GOT) using starch gel electrophoresis. Isozyme banding patterns showed a total of nine (9) presumptive loci (ACP-1, ACP-2, ALP-I, ALP-2, EST-I, EST-2, EST-3, GOT-I, and GOT-2). The degree of genetic variability was determined by estimating the proportion of polymorphic loci (P), average number of alleles per locus (A), average heterozygosity (H), genetic identity (IN), genetic distance (D), and genotypic similarity (IH). P. sajor-caju obtained the highest P, A, and H values of 0.429, 1.429, and 0.151, respectively. Both populations of V. volvacea species from Los Baños and Nueva Ecija obtained P, A, and H values of 0.250, 1.250, and 0.114, respectively. P. florida obtained the lowest values of P, A, and H (0, 1.0, 0). Based on these parameters, genetic variation was highest in P. sajor-caju and lowest in P. florida. IN (0.245 to 0.402), D (0.911 to 1.406), and 111(0.180 to 0.472) values obtained between Volvariella and Pleurotus genera suggested that these two genera were genetically different from each other. The computed IN, III, and D values when the two V. volvacea species (1.0, 0, and 0.981, respectively), and P. florida and P. sajor-caju species (0.798, 0.226, and 0.653, respectively) were compared indicate lesser variation between them.

Location

UPLB Main Library Special Collections Section (USCS)

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Document Type

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