Isozyme marker for resistance in leafhopper (Amrasca bigutulla ishida) in eggplant (Solanum melongena Linn.).

Date

3-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

To identify isozyme marker for resistance to leafhopper (Amrasca biguttula Ishida) in eggplant (Solanum melongena Linn.), protein samples were collected from the leaves of 11 eggplant accessions (four are resistant, six are moderately resistant, and one is susceptible). Isozyme analysis using 12 enzymes was done through starch gel electrophoresis. Eight enzymes namely acid phosphatase (ACP), alkaline phosphatase (ALP), esterase (EST). glucose-6-phosphate dehydrogenase (G6PD). isocitrate dehydro-genase (ICD), malate dehydrogenase (MDH). phosphogluconate dehydrogenase (PGD), and phosphoglucoisomerase (PGI) produced positive results. Relative mobility for each band was computed and banding patterns were established. The bands and banding patterns observed in ACP, ALPH. G6PD, ICD, MDH, PGD, and PGI failed to differentiate the resistant, moderately resistant, and susceptible varieties. Only the band for EST-1, observed in all resistant accessions. was significant at 5% level. This EST-1 locus may be used as an isozyme marker for resistance to leafhopper.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

This document is currently not available here.

Share

COinS