"Isozyme polymorphismin two orchid species: Vanda lamellata var. boxalli reich f. and Pomatocalpa bicolor (Lindley) J.J. Smith."

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Netzie N. Bebing

Abstract

Using starch gel electrophoresis, isozyme polymorphism in Vandu lamellata var. boxalli Reich f and Pomatocalpa bicolor (Lindley) J.J. Smith was analyzed based on seven enzymes that produced zone(s) of activity. The enzymes used were acid phosphatase (ACPH), phosphoglucomutase (PGIV1), esterase (EST), malate dehydrogenase (1v1D11), isocitrate dehydrogenase (IDH), glucose-6-phosphate dehydrogem.se (G6PD), and phosphoglucoisomerase (PGI). Isozyme banding patterns showed a total of eight presumptive loci. Only ACPH exhibited presence of two loci compared to the others that had only one locus. The degree of genetic variability between the two species was determined by computing the average number of alleles per locus (A), average heterozygozity (H), proportion of polymorphism (P), genetic identity (1), genetic distance (D) and genotypic similarity (I„y). Computed values for P were high in V. lamellata (50%) but low in P. bicolor (25%). It was estimated that there was one allele per locus (mean= 1.33036). The H values computed was both less than 50% (-21% only). Based on the values of H and P, more variation can be observed in V. lamellata than P. bicolor. Genetic identity for the two species observed was not that high (0.68) but significantly has very low genotypic identity or similarity (1„y) (0.051041). The value of D (0.39) between the two species of different genera was not that high and degree of similarity is low.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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