Morphological and cytological studies of three sugarcane (Saccharum officinarum L.) hybrids from the Philippines

Date

4-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

Morphological and cytological characteristics of three hybrids of sugarcane (Saccharum officinarum L.) from the Victoria Milling Company in Victoria, Negros Occidental, Philippines, namely VMC 87-557, VMC 89-76, and VMC 89-135 were observed and analyzed. Morphological data provided by NPGRL showed similarities in 25 out of 68 characteristics. VMC 87-557 and VMC 89-135 had the highest percentage of similarity (52.94%). This was followed by VMC 87-557 and VMC 89-76 (51.04%). VMC 89-76 and VMC 89-135 had the lowest percentage of similarity (47.06%). Variation was observed in 14 characteristics, the most notable of which were the outer auricle shape (deltoid in VMC 87-557, ascending transitional in VMC 89-76, straight transitional in VMC 89-135), inner auricle shape (deltoid in VMC 87-557, unciform in VMC 89-76, ascending transitional in VMC 89-135), dewlap secondary color (brown in VMC 87-557, greyed-purple in VMC 89-76, absent in VMC 89-135), color of the unexposed internode (greyed-orange in VMC 87-557, greyed-purple in VMC 89-76, brown in VMC 89-135), and bud shape (narrow ovate in VMC 87-557, round with central germ pore in VMC 89-76, roundish with wings in VMC 89-135). The three hybrids showed type V epidermal pattern since there were no paired cork-silica cells. Variation in chromosome configuration at diakinesis was observed among the three hybrids and within the same accession. The chromosome numbers with the highest frequency were 54 11 (2n=108) for VMC 87-557, 55 11 (2n=110) for VMC 89-76, and 49 11 (2n=98) for VMC 89-135. Meiosis was generally normal in all hybrids. The only abnormality observed was laggard formation in metaphase 1, metaphase 11, anaphase I, and anaphase 11. Metaphase I of VMC 87-557 had the highest incidence of laggards (25%).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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