Embryonic development of siamese fighting fish (Betta splendens, Regan) and the effect of Talisay leaf extract and tannic acid on the hatchability of eggs

Date

3-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Marni E. Cueno

Abstract

In an attempt to verify a putative acetyl-coenzyme A carboxylase (ACCase) involved in de two fatty acid biosynthesis in the plastids of coconut, 3' rapid amplification of cDNA ends (RACE) product was cloned and sequenced. The raw sequence data contained 140 nucleotide base pairs. The primer annealed completely which suggests that the putative ACCase was successfully cloned Translated sequences of frames +1, +2, and +3 were obtained, using CHROMAS program. Frame +1 was used in the analysis since it contains the longest coding region and the primer used was designed to start at frame +1. Analysis of the sequence further supported that the cloned gene was a possible ACCase. It contains the highly conserved Met-Lys-Met, which is the biotin-binding site of carboxylases, and leucine residue which suggests that it is a eukaryotic type of ACCase. BLASTX analysis was done on both undirected and directed ACCase. Undirected ACCase of BLASTX showed no significant similarity with known protein sequence database. However, 54 hits on the query sequence were obtained when BLASTX of directed ACCase was performed. The highest homologies were observed in Triticum aestivutn, Zea mays, Setaria Brassica napus, Alopecwws myosuroides and Glycine max. Their homologies were observed in the EVEVMKM region which contains the biotin binding site It can be seen that they belong to higher plant classes.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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