Comparizon of isozyme band patterns in endemic and indigenous species of Hoya in the Philippines.

Date

4-2005

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Co-adviser

Simeona V. Siar

Abstract

JUNIO, JULIUS JASON BIGCAS, April 2005. Comparison of Isozyme Band Patterns in !Endemic and Indigenous Species of Hoya in the Philippines. University of the Philippines Los Banos. Undergraduate Thesis. BS Biology major in Genetics.

Advisers: Dr Ma Genaleen Q. Diaz and Dr. Simeona V. Siar

ABSTRACT

Isozyme band patterns of eleven Hoya species and variants namely H. cilicaa, H. crassicaulis, H. cumingiana, H. densifolia, H. incrassaia, H. merrilli, H. meliflua, H. mindorensis, H. obscura and the 2 variants of H. pubicalyx were compared and analyzed using starch gel electrophoresis. A total of seventeen putative loci were found — one in phosphoglucoisomerase, four in esterase and acid phosphatase and two each in phsphoglucomutase, alkaline phosphatase, shikimate dehydrogenase and malic enzyme isozyme polymorphism determined the differences between the species based on the presence and absence of bands and their genotypic designation. Sixteen polymorphic putative loci were identified - one in phosphoglucoisomerase, two in phosphoglucomutase, shikimate dehydrogenase, malic enzyme, and alkaline phosphatase, three in esterase and four in acid phosphatase. Only a single locus was found to be monomorphic (SKD1). A 0.94 degree of polymorphism was obtained from the samples based on their genotypes and banding patterns. Using the combination of seven putative loci (ACP1/ACP2/ACP3/ACP4/( ALP1/ALP2/(EST1/EST2/EST3/(ME1/ME2/(PGI1/(PGM 1/ PGM2/(SKD1/SKD2), the indigenous and endemic Philippine Hoya species were genetically differentiated from each other based on their genotypic designations. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) was used to establish the relationship of the indigenous and endemic species of Philippine Hoyas through the presence or absence of bands. Hoya species whose isozyme banding patterns were alike was clustered together to indicate their genotypic similarity. The H. pubicalyx variants had a similarity index of 1 indicating that no difference was observed in the handing patterns using the seven enzyme system. The remaining Hoya species showed variations in genotypic banding patterns.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS