Morpho-anatomical, phytochemical and cytotoxicity studies on selected local varities of Lagundi, Vitex negundo L. (Lamiaceae)

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lourdes B. Cardenas

Abstract

Samples of four different accessions of lagundi (Vitex negundo L.) from NPGRL-IPB, UPLB showed differences in the leaf morphology, flower color and trichome density. Phytochemical investigation by thin layer chromatography (TLC) revealed differences in their essential oil, flavonoid and alkaloid profiles while their iridoid profiles were similar. First reported by Masilungan et al. (1971), cytotoxic activity of leaf extracts of lagundi was verified through brine shrimp lethality assay. Me0H extracts of all varieties exhibited high toxicity (LC50 = 20 - 60 ppm) towards brine shrimp nauplii while Sample 4 exhibited the highest toxicity among the EtOAc extracts (LC50 = 49 ppm). Trypan blue exclusion cytotoxicity assay, conducted at BRSL-NSRI, UPD, confirmed low cytotoxic activity toward human lymphocytes (IC50 = 262 ppm). Prolonged exposure to the plant extracts did not significantly affect cytotoxicity results. Samples 1, 2 and 3 were identified to be V. negundo while Sample 4 was identified to be V. trifolia. Varieties of V. negundo revealed wide range of morphological features, distinctive phytochemical constituents yet similar cytotoxic activity.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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