Genetic divergence among five species of Capsicum (Solanaceae) based on seed protein electrophoresis

Date

10-1993

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M. Engle

Abstract

Two hundred one accessions of Capsicum belonging to the five species: C. annuum, C. baccatum, C. chacoense, C. chinense, and C. trutescens, sampled from 48 countries were analysed. the seed proteins were extracted and resolved using sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE) with a 4% stacking and 12% resolving gel. Twelve bands were resolved, eight of which did not exhibit polymorphism. Seven band patterns were identified, with the highest frequency observed for band pattern 4 followed by band pattern 7. Among the species, C. baccatum was noted to exhibit the greatest number of band patterns while C. chinese the least. Computation of the mean infra- and interspecific similarity index showed close association between C. annuum, C. chinense, and C. frutescens. C. annuum exhibited a very low frequency of band 1 while C. baccatum the opposite. There is a possibility therefore to characterize the Iwo species using this particular band. The frequency distribution of the band patterns in the 10 assigned regions indicated that six out of the seven band patterns occurs in Western and Central Europe. Gene combinations thus is highest in the said region. On the other hand, the frequencies of the specific bands suggest that it is in South America that the genetic diversity is highest as the polymorphic bands have frequencies far from being fixed or being extinct.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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