Genetic diversity of seed proteins in population of mungbean, Vigna radiata (L.) Wilczek, from the Philippines using SDS-page

Date

10-1993

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M. Engle

Abstract

The study was conducted to test the hypothesis that genetic diversity in seed proteins exists in the mungbean germplasm originating from the Philippines, using SDS-PAGE. Its objectives were to determine the SDS-PAGE band-pattern distribution in the collection, to calculate the relative frequency of specific bands, and to determine how SDS-PAGE can be used in the management of germplasm collections. Initial results revealed five protein profiles; Profiles 4 and 5 shared low Jaccard similarity values with respect to Profile I, the predominant profile. Profile 4 belonged to V. glabrescens and Profile 5 belonged to "Red Mungbean," which was re-identified to be non-V. radian, based on plant and seed morphology. Only Profiles 1, 2 and 3 were thus analyzed. Profile I was exhibited by 98% of the 271 mungbean accessions. It was composed of the following bands: Band 1 (60.8 kD), Band 2 (57.6 kD), Band 3 (49.2 kD), Band 4 (43.7 kD), Band 5 (37.8 kD), Band 6 (34.0 kD), Band 7 (29.4 kD), Band 9 (23.7 kD), Band 10 (19.7 kD) and Band 11 (15.4 10). Profile 2 was exhibited by 1% of the accessions. It was composed of all the Profile I bands except for Band 9. Profile 3 was exhibited by 1% of the accessions. It was composed of all the Profile I bands plus Band 8 (26.9 kD). The predominance of Profile 1 among the accessions examined indicated a low degree of variation with respect to seed protein profiles. All bands exhibited a relative frequency of 1.00; except for Band 8 which exhibited 0.01 and Band 9 which exhibited 0.99. The bands were thus monomorphic with respect to a 0.99/0.01 polymorphism criterion, indicating low genetic variation with respect to seed protein polypeptides. SDS-PAGE of seed proteins was demonstrated to be useful in rapid identification of different species in a germplasm collection. It was able to identify non-V. radiata species that have been mistakenly assigned to the V. radiata collection. Characterization of the mungbean accessions based on seed protein profile was also demonstrated to be useful as an adjunct to established morphological cluster analysis, especially for the accessions that exhibited rare SDS-PAGE profiles. Thus, SDS-PAGE may be used to characterize genetic diversity of mungbean accessions with the final objective of nominating accessions to a core collection.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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