Genetic characterization of four Philippine native orchids: spathoglottis plicata 'Álba' blume, spathoglottis plicata blume, vanda lamelatta lindley, and cymbidium sp. through isozyme analysis .

Date

4-2012

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Co-adviser

Carmina C. Manuel

Abstract

Isozyme polymorphism in four Philippine native orchids, namely; Spathoglottis plicata 'alba Blume, Spathoglottis plicata Blume, Vanda lamellata Lindley and Cymbidium sp., was done using the following enzymes - acid phosphatase (ACP), alkaline phosphatase (ALP), esterase (EST), malate dehydrogenase (MDH) and glutamic oxaloacetic transaminase (GOT). Results showed eight presumptive loci for the enzyme systems. Two loci were observed each for ACP and ALP, three for EST, none for MD1-1, and one for GOT. Genetic variation was estimated by determining the proportion of polymorphic loci (P), average number of alleles per locus (A), and the average heterozygozity (H), genetic identity (IN), genetic distance (D), and genotypic similarities (IH). S. plicata 'alba' exhibited the highest proportion of polymorphic loci (87.50%), followed by V. lamellata (83.33%), S. plicata (62.50%) and Cymbidium sp. (57.14%). There were approximately two alleles observed for each locus. Values for average heterozygosity ranged only from 19.35% - 47.19%, suggesting low genetic variability. On the other hand, values for genetic identity and genotypic similarity ranged from 0.5046 - 0.8317 and 0.2602 - 0.4703, respectively. The highest value of genetic identity (0.8317) and genotypic identity (0.5488) was observed between S. plicata 'alba' and Cymbidium sp. These species also exhibited the lowest genetic distance (0.1843) even though they belong to different genera. This finding suggests that the enzyme systems used may not have been able to fully describe variation between and among the species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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