Preliminary study on DNA barcoding of domesticated pig breeds/strains (Sus scrofa domesticus)

Date

4-2013

Degree

Bachelor of Science in Agriculture

Major Course

Major in Animal Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Orville L. Bondoc

Abstract

The study sought to generate DNA barcodes (i.e. cytochrome c oxidase subunit I or CO1 of the mitochondria) genome) for five domesticated swine breeds (i.e. Duroc, Landrace, Large White and Native pigs from Kalinga and Quezon provinces). The COI sequences were analyzed for genetic diversity, distance, and relationships using the Neighbour-Joining (NJ) method based on Kimura 2-parameter model in Molecular Evolutionary Genetics version 5 (MEGA5). Based on 633 COI positions, the overall genetic diversity of the sampled swine breeds/strains was 17.58 ± 1.29A. Average pair-wise distance within taxa was highest between Large White and Duroc (0.210 0.020 d-units) and lowest between Large White and Landrace (0.125 0.014 d-units). The genetic distance between the native pigs from Kalinga and Quezon provinces was 0.202 ± 0.019 d-units. The rooted NJ tree showed that Kalinga native pig is distinctly different from the European white breeds (i.e. Landrace and Large White) and the Dame and Kalinga native pig. More COI s equences for commercial purebreds, crossbreeds, and native pigs are recommended to be determined in the future. Large dataset will increase the reliability of DNA barcoding to identify or discriminate pig breeds/strains. Also, it will enable to check if there are earlier erroneous submissions that will be corrected in the future.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2013 A3 D66

Document Type

Thesis

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