Molecular characterization of selected BC2F1 individuals of Abaca(Musa Textilis Nee) for tensile strength using microsatellite markers.

Date

4-2012

Degree

Bachelor of Science in Biology

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Antonio G. Lalusin

Abstract

To determine microsatellite markers that are possibly associated with tensile strength, selected BC2Ft individuals of abaca (Musa textilis Nee), Pacol and Abuab were subjected to PCR using 16 Musa-based SSR primers. Among the selected BC2F1 individuals, five possessed superior tensile strength ranging from 17.80 to 26.33 kg/g/m while five samples have low tensile strength ranging from 14.25 to 16.82 kg/g/m. Seven markers, namely: MOSAT 39, MOSAT 150, MOSAT 1-12, MOSAT 22, MOSAT 264, MOSAT 1113 and MOSAT 25 were found to identify polymorphism. Among the seven polymorphic markers, only three markers (MOSAT 39, MOSAT 1-12 and MOSAT 264) were able to produce bands unique to the samples having a superior tensile strength. These bands were possibly associated with tensile strength of abaca hybrids and can be used in assessing tensile strength in BC2 F1 population. These three markers detected a high number of alleles compared to the others; there were four for MOSAT 39 and three each for MOSAT 1-12 and MOSAT 264. All three markers had major allele frequency of 0.50 and heterozygosity of 0.08. In terms of gene diversity, MOSAT 39 obtained a value of 0.60, 0.62 for MOSAT 1-12 and 0.54 for MOSAT 264. The polymorphic information content (PIC) value of MOSAT 39 is 0.52, 0.55 for MOSAT 1-12 and 0.43 for MOSAT 264.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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