Isozyme polymorphism in the Philippine orchids: Vanda luzonica Loher ex Rolfe, Vand lamellata Lindl. and Aerides spp.

Date

10-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita L. Rosario

Abstract

Isozyme patterns for alkaline phosphatase (ALP), acid phophatase (ACP), malate dehydrogenase (MDH) and esterase (EST) were used to detect isozyme polymorphism in 10 orchid cultivars namely Vanda luzonica Loher ex Rolfe, Vanda lamellata Lindl, , V. lamellata var. boxalli Reichb.f. , V. lamellata var. calayana Valmayor and Tiu, V. lamellata var. flava Valmayor and Tiu Vanda lamellata var. remediosae Ames and Quis, Aerides jarkiana Schltr., A. lawrenceae Reichb.f., A. odorata (Poir.) Lour, and A. quinquevulnera Lindl. by using horizontal starch gel electrophoresis. Isozyme polymorphism determined differences among varieties and species based on the presence or absence of bands and genotype designation. Various isozyme profiles revealed that all Vanda varieties and species possess striking similarity. All Aerides species also exhibited similar genotypes which were different with those of the Vanda cultivars. Two out of 8 loci were found to be polymorphic. A 0.25 degree of polymorphism and an average heterozygosity of 0.1 were obtained from the 10 accessions based on their genotypes and banding patterns. The low genetic values indicate that cultivars exhibited close similarity and relationship since they belong to the same family Orchidaceae and only differed in genus where each variety belongs.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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