The potential of bitter gourd (Momordica charantia L.) as biological against Aspergillus niger L. and Rhizopus nigricans L.

Date

4-2003

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Nina M. Cadiz

Abstract

A study was conducted to assess the potential of bitter gourd (Momordica chat-antic, L.) as a biological control for Aspergillus niger L. and Rhizopus nigricans L. Two bioassay techniques: (1) bioassay using the swabbing method and (2) incorporation of the extracts to the PDA media, were evaluated. The effects of different concentrations (0, 25%, 50%, 75%, and 100%) of fruit-/leaf-water extracts and fruit-/leaf-ethanol extracts were studied. The first method revealed that leaf extracts can better inhibit the growth of A. niger and R. nigricans compared to fruit extracts. Leaf extracts (either water-or ethanol-extracted) caused larger zones of inhibition (e.g. 9.33 mm vs. 7.67 mm) than that caused by the fruit extracts, although significant difference was only between the ethanol-extracted fruit/leaf. Further, the swabbing method revealed better growth inhibition of Aspergillus niger in fruit-/leaf-ethanol extract than in fruit-/leaf-water extract. Results of the second method (i.e. incorporation of the ampalaya extracts to the media) further support the findings in the first method that leaf-ethanol extracts of ampalaya significantly inhibited the growth of the fungi as compared with leaf-water extract. Therefore, the two methods reinforced each other's findings that in general, leaf-ethanol extract can better inhibit the growth of the fungi compared to leaf-water extract.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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