Cross-amplification of microsatellite loci using Simple Sequence Repeats (SSR) primers developed from four selected endemic Philippine goby species.

Date

4-2012

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

Ten Simple Sequence Repeats (SSR) primer pairs designed from sequences derived from microsatellite-enriched DNA libraries of four Philippine endemic goby species namely, Glossogobius celebius, G. giuris, Gobiopterus lacustris and Rhinogobius giurinu, were used to cross-amplify some SSR loci. Eight of the ten SSR primers were able to amplify SSR loci in all four species. Primer Gi21 amplified SSR loci in two of the four species, while primer Gi51 amplified SSR loci in three species. Generally, the designed SSR primers were able to amplify not only the SSR loci of the species from which they were derived from but also those of other species. A total of 58 alleles were generated from the 10 SSR primers. Primers Gc39, GIaCT, and Rh18 amplified the highest number of alleles (8) while primer GIaGA amplified the lowest (3). Among the loci amplified by the 10 SSR primers tested against four species the Polymorphic Information Content (PIC) values ranged from 0.4316 to 0.8142 with an average of 0.6691. Eighty percent of the amplified loci were considered highly informative. At a relatively low level of similarity (39%), the constructed dendrogram showed five clusters and indicate that G. celebius and G. lacustris are probably more similar.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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