Inheritance of two enzyme systems of Zea mays (I12), Zea diploperennis (Acc 167) and their F1, F2, and BC1 hybrids.

Date

4-1994

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dolores A. Ramirez

Abstract

The inheritance of two enzyme systems, isocitrate dehydrogenase (IDH) and 6-phosphogluconate dehydrogenase (6-PGD) of it, (I.,) and diploperennis (Ace 167) and their F., F, and BC, hybrids was determined using polyacrylamide gel electrophoresis. Relative mobilities of the bands expressed and the number of bands per plant were observed. Only one zone of activity was observed for each of the two isozymes in all the populations studied. Two banding patterns were resolved within the observed zone of activity. Maize and the diploperennis parent were similar in terms of the relative mobility of the IDH but difference in the Rf of PGD was noted. Combination of the two fast bands coming from the two parents was resolved in all FL plants indicating a possible gene transfer from the wild relative to the cultivated maize. Segregation of the individuals in terms of the two isozymes fitted the expected frequency for independently segregating alleles. On the other hand, more plants in the BC. population were noted to have similar banding patterns with that of the maize parent in terms of PGD while a combination of the fast band of the maize parent and the slow band of the diploperennis parent were observed in the IDH banding pattern. Heterozygosity and polymorphism were observed for both loci indicative of the large amount of genetic variability within the genome of both parents: mays and Zea diploperennis

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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