Electric comparison of green leafhopper (GLH) Nephotettix virescens (Distant) sampled from tungro-infested and tungro non-infested rice fields in Bay, Laguna

Date

4-1996

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

A study was conducted to analyze and compare the electrophoretic banding patterns for esterase, glucose-6-phosphate dehydrogenase, and malic enzyme of N. virescens males sampled from tungro-infested and tungro non-infested rice fields of Hangan and Sto. Domingo, Bay, Laguna. Homogenates of body tissues of N. virescens were utilized in starch gel electrophoresis. Results showed that glucose-6-phosphate dehydrogenase is the potential genetic marker, for differentiating N. virescens populations from tungro-infested and tungro non-infested rice fields. In terms of the collection site glucose-6-phosphate dehydrogenase of N. virescens from tungro infested rice fields in Hangan was significantly higher in Rf value than that of Sto. Domingo, which can be correlated with the severity of tungro infestation. Est phenotypes of N. virescens from tungro non-infested rice fields have gene frequencies of 0.6 and 0.4 for Hangan and 0.52 and 0.48 for Sto Domingo. While N. virescens from tungro-infested rice fields with gene frequencies of 0.38 and 0.62 for Hangan, and 0.58 and 0.42 for Sto. Domingo. G-6-PD phenotypes of N. virescens from tungro non-infested rice fields have gene frequencies of 0.45 and 0.55 for Hangan 0.3 and 0.7 for Sto. Domingo. While for N. virescens from tungro-infested rice fields with gene frequencies of 0.57 and 0.43 for Hangan, and 0.55 and 0.45 for Sto. Domingo. Malic enzyme phenotypes of N. virescens from tungro non-infested rice fields have gene frequencies of 0.37 and 0.63 for Hangan and 0.42 and 0.58 for Sto. Domingo. While for N. virescens from tungro-infested rice fields with gene frequencies of 0.38 and 0.62 for Hangan, and 0.6 and 0.4 for Sto. Domingo. Est, G-6-PD and Malic Enzyme appears to be controlled by a single locus with two alleles. Chi-square test of the genotype frequencies showed Est loci from N. viresccens from tungro non-infested and tungro-infested rice fields at Hangan and sto. Domingo, showed a goodness-of-fit to expected frequencies under the Hardy-Weinberg equilibrium. Those which deviated from the expected proportions may have undergone non-random mating and could be affected by migration and selection pressure in the populations. The genetic identity (I) was high and genetic distance (D) was low which further tells that the two populations of N. virescens are genetically related and their genetic characters are almost the same. The two populations could have come from only one population.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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