Comparison of glucose-6- phosphate dehydrogenase isozymes between diabetics and non-diabetics

Date

3-1991

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Two glucose-6-phosphate dehydrogenase isozymes were detected in thirteen IDDM, eighty NIDDM and thirty four non-diabetics from five sampling areas. (1(TD1, with mean Rf value of 0.4808 was most frequent. in IN,M. G6PD2, with 0.139 mean Rf value was most frequent in the non-diabetics group. A faint DH band, probably a dehydrogenase enzyme, was observed only among NIDDM with a frequency of 10.83 percent. Three isozyme band patterns were ,served bands GGPD1/G6PD1, G6PD2/G6PD2 and G6PD1/G6PD2 double band, in NIDDM and non-diabetics. G6PD1/G6PD2 was nut however observed in IDDM. The frequencies of the banding pattern,- in NIDDM sampling areas varied considerably and between male and female subjects. GGPD1/G6PD2 in female subjects. The frequencies of the G6PD genotypes do not follow the Hardy-Weinberg equilibrium probably due to non=random sampling of the diabetic subject The ranges decolorization time varied among the three groups. NIDDM ranged from 15=60 minute, IDDM ranged from' 30-t11 minutes. Non-diabetics showed the widest range 15-75 minutes. There was a slight correlation between phenotype and the activity of the enzyme.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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