DNA fingerprinting of mango fruit fly, Bactrocera philippinensis Drew and Hancock, using random amplified polymorphic DNA.

Date

3-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

The genetic profile and genetic variation of 15 males from a reared population of mango fruit fly, Bactrocera philippinencis Drew and Hancock were analyzed using. RAPD-PCR technique. The eight ten-mer primers used produced different fragment sizes. Seven out of eight primers generated different banding patterns for the 15 individuals which could probably be attributed to the intrinsic genetic differences between them and could also suggest a high degree of heterogeneity. On the other hand, with primer 4. seven individuals showed similar handing patterns which possibly mean that this primer can not fully detect the genetic variability of the insects. 1 he total number I,:i observed was 101 in which 94.06% was polymorphic. Monomorphic loci include P: i 5. P3-2, P4-10, P6-12 generated by primers 1, 3, 4 and 6, respectively and also P8-2 P8-5 amplified through primer 8. Gene frequencies for each locus were computed the POPGENE 32 software. Nei's genie variation was low ranging from 0 to 0.5 at 0 813 indicating that the individuals in the studied population were closely related to each other.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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