Genetic variation within a population of Philippine mallard duck (Anas platyrhynchos, L.) sampled from Pila, Laguna using microsatellite-PCR analysis.

Date

5-2009

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

The genetic variation within a population of Philippine Mallard duck (Anas plalyrhynchos, L.) from Pila, Laguna was analyzed using eight microsatellite markers, namely: APHI4, SMO6, SMO7, SMO9, SM010, SM011, SM012, and SM013. DNA was extracted from duck blood samples in FTA cards and was subjected to polymerase chain reactions (PCR). PCR products were visualized using agarose gel electrophoresis (AGE) and polyacrylamide gel electrophoresis (PAGE). The optimized conditions were established as 0.2 and 0.4 U for Tag polymerase concentration and 2.0 and 5.0 ill sample volume. Only SMO6, SMO7 and SM011 were found to be polymorphic. Two to three alleles (A, B, C) were detected per locus. The overall mean gene diversity was 0.5410, indicating that homozygosity within the population was high. Band sharing estimates of the alleles with a mean value of 0.79 implying that there was high similarity among the alleles observed in each locus supported this. Average general observed heterozygosity of 0.3500 further showed that there was heterozygote deficiency within the population per locus considered. Moreover, the computed polymorphic information content (PIC) of each locus was relatively low: 0.21875 for SMO6, 0.33875 for SMO7 and 0.42000 for SM011. Substantial sample uniformity was also seen wherein one group possessed a genotypic pattern sharing of 25%. The overall low diversity observed in the population studied may be attributed to unsystematic breeding of ducks and the small sample size analyzed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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